Activity Screening of Bacteria Containing Renilla Luciferase Plasmids
نویسندگان
چکیده
منابع مشابه
PAST PROJECTS Mechanical control of Renilla Luciferase
Enzymes are deformable molecules: the molecular structure can exist in essentially a continuum of different conformations. These are accessible by applying mechanical stress to the molecule. Mechanics then provides a general molecular mechanism to modulate enzymatic reactions, by applying mechanical stresses which deform the enzyme. There are approximately 10 known different enzymatic reactions...
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The luciferase protein fragment complementation assay is a powerful tool for studying protein-protein interactions. Two inactive fragments of luciferase are genetically fused to interacting proteins, and when these two proteins interact, the luciferase fragments can reversibly associate and reconstitute enzyme activity. Though this technology has been used extensively in live eukaryotic cells, ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98242bm02